Journal: Experimental hematology

Article Title: CLL-like monoclonal B cell lymphocytosis displays an increased inflammatory signature that is reduced in early stage chronic lymphocytic leukemia

doi: 10.1016/j.exphem.2020.12.007

Figure Lengend Snippet: Flow cytometry gating strategy. FVS510 was used to select viable cells (1). After gating for singlets (2), CD45+CD14+CD4+ cells (monocytes) were selected (3 and 4) and linear mean fluorescence intensity (MFI) values of HLA-DR were registered (5a). The absence of fluorescence for PE-Cy7 on monocytes was confirmed employing isotype control (5b). On the other hand, CD3+CD4+ cells (CD4+ T cells) were gated (6). The percentage of CD4+ T cells that were positive for CXCR3 (7a) and PD1 or negative for CD27 was assessed employing isotype controls (7b), whereas the percentage of CD4+ T cells that were positive for perforin (8a) and granzyme B was evaluated using fluorescence-minus-one (FMO) controls (8b). In the plots, fluorescence intensity values are transformed into logarithmic scale.

Article Snippet: Additionally, our MBL group displayed the higher median percentage of effector CD27 − CD4 + T cells.

Techniques: Flow Cytometry, Fluorescence, Control, Transformation Assay

Journal: Experimental hematology

Article Title: CLL-like monoclonal B cell lymphocytosis displays an increased inflammatory signature that is reduced in early stage chronic lymphocytic leukemia

doi: 10.1016/j.exphem.2020.12.007

Figure Lengend Snippet: Flow cytometry analysis. MFI of HLA-DR was assessed on monocytes (A), and the percentages (%) of CD4+ T cells that were positive for CXCR3 (B), perforin (C), granzyme B (D), PD1 (E) or negative for CD27 (F) were registered. Significant P-values are indicated with * (<0.05), ** (<0.01) or *** (<0.001). CTR: Healthy controls.

Article Snippet: Additionally, our MBL group displayed the higher median percentage of effector CD27 − CD4 + T cells.

Techniques: Flow Cytometry